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BLOOD GROUPING TEST

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Rhofinal Anti-D

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Rhofinal Anti-D
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Product Details:
Packaging Size6 x 10ml
BrandRhofinal
Grade StandardReagent Grade
Packaging TypeBox
ApplicationReagent for routine typing and confirmation of Rho(D) phenotypes by slide and tube test
Titre>/- 1:128
Class of AntibodyIgM + IgG, Monoclonal Blend
SourceHuman B cell line, EBV transformed
Specificity100% to Rho(D) antigen
StandardisationFollows AABB and FDA guidelines
Storage / Stability2-8 Deg C, 24 months
ManufacturerTulip

SUMMARY
Monoclonal antibodies are derived from hybridoma cell lines, created by fusing mouse antibody producing B lymphocytes with mouse myeloma cells orare derived froma human B cell line through EBV transformation. Each hybridoma cell line produces homogenous antibodies of only one immunoglobulinclass, which are identical in their chemical structure and immunological activity.
Human red blood cells are classified as Rho (D) positive and Rho (D} negative depending upon the presence or absence of D (Rho) antigen on them.Approximately 85% of the Caucasian population are Rho (D) positive. The D’ phenotype is a traditional definition to describe the weak/ partial D's thatcanbe detected with Anti-D (Rho} (IgM+IgG).
About 60% of the D’(weak/ partial D's) may react with Anti-D (Rho} (IgM+lgG) in slide tests and about 90% may be detected by the tube technique.


REAGENT
RHOFINAL® Anti-D (Rho) {IgM + IgG) is a ready to use reagent, prepared from supematants of cell cultures with antibody producing B lymphocytesobtained through EBV transformation and is a blend of Agglutinating Sera of the immunoglobulin class IgM and IgG (CloneP3X61+NaTH119+LOR15C9}. These Agglutinating Sera are of the same specificity but having the capability of recognising different epitopes of thehuman red blood call antigen D (Rho).
RHOFINAL’ Anti-D (Rho) {IgM + IgG) is a blend of Agglutinating sera of IgM and IgG class which agglutinates the D (Rho) antigen, a characteristic whichaccords versatility to the reagent. It gives an avid saline reacting slide / tube test reagent the capability of detecting D’ (weak/partial D's) in the Anti HumanGlobulin Phase.

PRINCIPLE
Human red blood cell possessing D antigen will agglutinate in the presence of Agglutinating Sera directed towards the antigen. Agglutination of red bloodcells with RHOFINAL” Anti-D (Rho) {IgM + IgG) reagentis a positive test result and indicates the presence of the D (Rho) antigen. No agglutination withRHOFINAL® Anti-D (Rho) {IgM + IgG) reagentis a negative test result and indicates absence of D (Rho) antigen. All negative testrasults should be furthertested for D" (weak/ partial D's) by performing the D'test procedure, as described later.
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