BLOOD GROUPING TEST

Product Details:

Summary
Monoclonal antibodies are derived from hybridoma cell lines, created by fusing mouse antibody producing B lymphocytes with mouse myeloma cells. Each hybridoma cell line produces homogenous antibodies of only one immunoglobulin class, which are identical in their chemical structure and immunological activity. Human red blood cell antigens can be divided into four groups A, B, AB and O depending on the presence or absence of the corresponding antigens on the red blood cells. Approximately 41% of the Caucasian population have theAAntigen, 9% have the BAntigen, 4% have bothAand B antigens, while the remaining have neither the Anor the B antigen. 

Reagents
Eryclone Anti-A (Clone 11H5), Eryclone Anti-B (Clone 6F9), and Eryclone Anti-A,B (Clone 11H5+6F9) are ready to use solutions of the respective specific antibodies of the immunoglobulin class IgM prepared from the corresponding supernatants of mouse hybridoma cell cultures. Each batch of reagent undergoes rigorous quality control at various stages of manufacture for its specificity, avidity and performance.

Principle
Human red blood cells possessingAand/or B antigen will agglutinate in the presence of antibody directed towards the antigen.Agglutination of red blood cells with ® ® ® Eryclone Anti-A,Eryclone Anti-B,Eryclone Anti-A,B reagents is a positive test result and indicates the presence of the corresponding antigen.Absence of agglutination of red blood cells with Eryclone Anti-A, Eryclone Anti-B, and Eryclone Anti-A,B reagents is a negative test result and indicates the absence of the corresponding antigen.

Product Details:

Summary
Monoclonal antibodies are derived from hybridoma cell lines, created by fusing mouse antibody producing B lymphocytes with mouse myeloma cells. Each hybridoma cell line produces homogenous antibodies of only one immunoglobulin class, which are identical in their chemical structure and immunological activity. Human red blood cell antigens can be divided into four groups A, B, AB and O depending on the presence or absence of the corresponding antigens on the red blood cells. Approximately 41% of the Caucasian population have theAAntigen, 9% have the BAntigen, 4% have bothAand B antigens, while the remaining have neither the Anor the B antigen.

Reagents
Eryclone Anti-A (Clone 11H5), Eryclone Anti-B (Clone 6F9), and ERYCLONE Anti-A,B (Clone 11H5+6F9) are ready to use solutions of the respective specific antibodies of the immunoglobulin class IgM prepared from the corresponding supernatants of mouse hybridoma cell cultures. Each batch of reagent undergoes rigorous quality control at various stages of manufacture for its specificity, avidity and performance. 

Principle
Human red blood cells possessingAand/or B antigen will agglutinate in the presence of antibody directed towards the antigen.Agglutination of red blood cells with Eryclone anti-a,eryclone Anti B,eryclone Anti-A,B reagents is a positive test result and indicates the presence of the corresponding antigen. Absence of agglutination of red blood cells with eryclone Anti-A, eryclone Anti-B, and eryclone Anti-A,B reagents is a negative test result and indicates the absence of the corresponding antigen. 

Precautions
1. In vitro diagnostic reagent for laboratory and professional use only. To be used by a qualified personnel. Not for medicinal use.
2. The reagent contains sodium azide 0.1% as preservative.Avoid contact with skin and mucosa. MSDS available on request.
3. Extreme turbidity may indicate microbial contamination or denaturation of protein due to thermal damage. Such reagents should be discarded.
4. Reagents are not from human source, hence contamination due to HBsAg, HIV and HCV is practically excluded.
5. It is necessary to use the dropper provided in the reagent vial to dispense a reagent drop.
6. It is advisable to wear gloves and safety spectacles and handle test specimens of human origin with caution.
7. Do not use damaged or leaking reagents. 8. Special protective measures, conditions for disposal and disinfection should be implemented in accordance with local regulations.

Product Details:

SUMMARY
Monoclonal antibodies are derived from hybridoma cell lines, created by fusing mouse antibody producing B lymphocytes with mouse myeloma cells orare derived froma human B cell line through EBV transformation. Each hybridoma cell line produces homogenous antibodies of only one immunoglobulinclass, which are identical in their chemical structure and immunological activity.
Human red blood cells are classified as Rho (D) positive and Rho (D} negative depending upon the presence or absence of D (Rho) antigen on them.Approximately 85% of the Caucasian population are Rho (D) positive. The D’ phenotype is a traditional definition to describe the weak/ partial D's thatcanbe detected with Anti-D (Rho} (IgM+IgG).
About 60% of the D’(weak/ partial D's) may react with Anti-D (Rho} (IgM+lgG) in slide tests and about 90% may be detected by the tube technique.


REAGENT
RHOFINAL® Anti-D (Rho) {IgM + IgG) is a ready to use reagent, prepared from supematants of cell cultures with antibody producing B lymphocytesobtained through EBV transformation and is a blend of Agglutinating Sera of the immunoglobulin class IgM and IgG (CloneP3X61+NaTH119+LOR15C9}. These Agglutinating Sera are of the same specificity but having the capability of recognising different epitopes of thehuman red blood call antigen D (Rho).
RHOFINAL’ Anti-D (Rho) {IgM + IgG) is a blend of Agglutinating sera of IgM and IgG class which agglutinates the D (Rho) antigen, a characteristic whichaccords versatility to the reagent. It gives an avid saline reacting slide / tube test reagent the capability of detecting D’ (weak/partial D's) in the Anti HumanGlobulin Phase.

PRINCIPLE
Human red blood cell possessing D antigen will agglutinate in the presence of Agglutinating Sera directed towards the antigen. Agglutination of red bloodcells with RHOFINAL” Anti-D (Rho) {IgM + IgG) reagentis a positive test result and indicates the presence of the D (Rho) antigen. No agglutination withRHOFINAL® Anti-D (Rho) {IgM + IgG) reagentis a negative test result and indicates absence of D (Rho) antigen. All negative testrasults should be furthertested for D" (weak/ partial D's) by performing the D'test procedure, as described later.

Product Details:

Blood Typing antibodies (IgM) for Slide and Tube Tests