Latex Test

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SUMMARY
During coagulation sequence of reactions occurs in the body in response to variety of external and or intemal stimuli. Theenzymatic cascade reaction terminates in the conversion of FIBRINOGEN to FIBRIN, by the enzyme THROMBIN. The fibringelis then converted toa stable fibrin clot by thrombin activated Factor XIII.
Finally, the fibrin network is dissolved by the enzyme PLASMIN to generate cross-linked fibrin degradation products (TULIPXL FDP*). D dimer comprising of two D fragments cross linked together, is the smallest plasmin resistant molecular unitpresentwithin TULIP XL FDP*®.
Detection of D dimer is invaluable as a diagnostic marker for thrombotic conditions such as DIC, DVT and PE. D dimer levelscan also be used to monitor thrombolytic therapy with t-PA and with streptokinase, thrombotic complications in pregnancy,acute myocardial infarction, sickle cell crisis, severe septic infections, liver disease, DIC accompanying snake bite andprognosis and response to therapy in cancer.


PRINCIPLE
TULIP XL FDP? slide test for detection of cross-linked fibrin degradation products is based on the principle of agglutination.The test specimen (plasma) is mixed with TULIP XL FDP” latex reagent. The sensitivity of the reagent is « 200 ng/ml, below,which samples are negative and above which samples give a positive agglutination reaction.
The cross-linked fibrin degradation products, D dimer, D dimer E, and high molecular weight derivatives are all recognized byTULIP XL FDP® reagent incorporating the agglutinating sera. No binding was found to the fibrinogen degradation products X,Y, D, and E to 20 mg/L or to fibrinogen upto 1000 mg/L.

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SUMMARY
Sometimes autoantibodies are produced by the human body against self antigens. The precise role that this aberrantimmunity plays in the pathogenesis of certain rheumatic diseases is unknown. However the presence of these autoantibodiesserves as credible marker of the disease.
In rheumatoid arthritis, diagnostically useful autoantibodies termed as "Rheumatoid factors" (RF) can be detected which areimmunoglobulins of the class IgM, IgG, IgA and IgE. Practically, IgM class RF with specificity to human IgG (Fc) is the mostuseful prognostic marker of RA. The clinical significance of RF determinations consists in differentiation between rheumatoidarthritis, in which RF of modified IgM class have been demonstrated in the serum of approximately 80% of the cases examinedand rheumatic fever, in which RF are almost always absent. The agglutination test is most frequently used because of itsgreater sensitivity and simplicity.


REAGENTS
1. RHELAX®-RF reagent (latex): A uniform suspension of polystyrene latex particles coated with suitably modified Fcfraction of IgG {agglutinating sera). The reagent is standardised to detect +10 IU/ml of RF or more. The standardization ofdetection limit of RHELAX*-RF is traceable to the W.H.O., 1st International Reference Preparation of RheumatoidArthritis Serum.
2. Positive control, reactive with the RHELAX*-RF reagent.
3. Negative control, non-reactive with the RHELAX*-RF reagent.

PRINCIPLE
RHELAX*-RF slide test for detection of rheumatoid factors is based on the principle of agglutination. The test specimen ismixed with RHELAX’-RF latex reagent and allowed to react. If RF is present within detectable levels then a visibleagglutination is observed. If RF is absent below detectable levels then no agglutination is observed.

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